Skip Navigation



JXB Advance Access published online on October 30, 2006
Journal of Experimental Botany, doi:10.1093/jxb/erl170
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
57/15/4003    most recent
erl170v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Roth, U.
Right arrow Articles by Clemens, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Roth, U.
Right arrow Articles by Clemens, S.
Agricola
Right arrow Articles by Roth, U.
Right arrow Articles by Clemens, S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author [2006]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org
Received May 26, 2006
Accepted August 24, 2006

RESEARCH PAPER

Proteome changes in Arabidopsis thaliana roots upon exposure to Cd2+

Udo Roth 1 *, Edda von Roepenack-Lahaye 2 *, and Stephan Clemens 2 *

1 Leibniz Institute of Plant Biochemistry, Halle (Saale), Germany; Present address: QIAGEN GmbH, Hilden, Germany
2 Leibniz Institute of Plant Biochemistry, Halle (Saale), Germany

* To whom correspondence should be addressed.
Stephan Clemens, E-mail: sclemens{at}ipb-halle.de


  Abstract

Cadmium is a major environmental pollutant that enters human food via accumulation in crop plants. Responses of plants to cadmium exposure--which directly influence accumulation rates--are not well understood. In general, little is known about stress-elicited changes in plants at the proteome level. Alterations in the root proteome of hydroponically grown Arabidopsis thaliana plants treated with 10 µM Cd2+ for 24 h are reported here. These conditions trigger the synthesis of phytochelatins (PCs), glutathione-derived metal-binding peptides, shown here as PC2 accumulation. Two-dimensional gel electrophoresis using different pH gradients in the first dimension detected on average ~1100 spots per gel type. Forty-one spots indicated significant changes in protein abundance upon Cd2+ treatment. Seventeen proteins found in 25 spots were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Selected results were independently confirmed by western analysis and selective enrichment of a protein family (glutathione S-transferases) through affinity chromatography. Most of the identified proteins belong to four different classes: metabolic enzymes such as ATP sulphurylase, glycine hydroxymethyltransferase, and trehalose-6-phosphate phosphatase; glutathione S-transferases; latex allergen-like proteins; and unknown proteins. These results represent a basis for reverse genetics studies to better understand plant responses to toxic metal exposure and to the generation of internal sinks for reduced sulphur.

Keywords: Cadmium; heavy metal accumulation; heavy metal tolerance; MALDI-TOF-MS; phytochelatins; proteomics.

*These authors contributed equally to the work.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Exp BotHome page
Y. Jiang, B. Yang, N. S. Harris, and M. K. Deyholos
Comparative proteomic analysis of NaCl stress-responsive proteins in Arabidopsis roots
J. Exp. Bot., October 4, 2007; (2007) erm207v1.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.