Skip Navigation



JXB Advance Access published online on November 6, 2006
Journal of Experimental Botany, doi:10.1093/jxb/erl199
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
57/15/4257    most recent
erl199v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Kader, Md. A.
Right arrow Articles by Lindberg, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kader, Md. A.
Right arrow Articles by Lindberg, S.
Agricola
Right arrow Articles by Kader, Md. A.
Right arrow Articles by Lindberg, S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author [2006]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org
Received August 15, 2006
Accepted September 13, 2006

RESEARCH PAPER

Expressions of OsHKT1, OsHKT2, and OsVHA are differentially regulated under NaCl stress in salt-sensitive and salt-tolerant rice (Oryza sativa L.) cultivars

Md. Abdul Kader 1 *, Thorsten Seidel 1, Dortje Golldack 2, and Sylvia Lindberg 1

1 Department of Plant Biology and Forest Genetics, Swedish University of Agricultural Sciences, Box 7080, SE 75007 Uppsala, Sweden
2 Department of Biochemistry and Physiology of Plants, University of Bielefeld, D-33501 Bielefeld, Germany

* To whom correspondence should be addressed.
Md. Abdul Kader, E-mail: abdul.kader{at}vbsg.slu.se


  Abstract

Under NaCl-dominated salt stress, the key to plant survival is maintaining a low cytosolic Na+ level or Na+/K+ ratio. The OsHKT1, OsHKT2, and OsVHA transporter genes might play important roles in maintaining cytosolic Na+ homeostasis in rice (Oryza sativa L. indica cvs Pokkali and BRRI Dhan29). Upon NaCl stress, the OsHKT1 transcript was significantly down-regulated in salt-tolerant cv. Pokkali, but not in salt-sensitive cv. BRRI Dhan29. NaCl stress induced the expression of OsHKT2 and OsVHA in both Pokkali and BRRI Dhan29. In cv. Pokkali, OsHKT2 and OsVHA transcripts were induced immediately after NaCl stress. However, in cv. BRRI Dhan29, the induction of OsHKT2 was quite low and of OsVHA was low and delayed, compared with that in cv. Pokkali. OsHKT2 and OsVHA induction mostly occurred in the phloem, in the transition from phloem to mesophyll cells, and in the mesophyll cells of the leaves. The vacuolar area in cv. Pokkali did not change under either short- (5-10 min) or long-term (24 h) salt stress, although it significantly increased 24 h after the stress in cv. BRRI Dhan29. When expressional constructs of VHA-c and VHA-a with YFP and CFP were introduced into isolated protoplasts of cvs Pokkali and BRRI Dhan29, the fluorescence resonance energy transfer (FRET) efficiency between VHA-c and VHA-a upon salt stress decreased slightly in cv. Pokkali, but increased significantly in cv. BRRI Dhan29. The results suggest that the salt-tolerant cv. Pokkali regulates the expression of OsHKT1, OsHKT2, and OsVHA differently from how the salt-sensitive cv. BRRI Dhan29 does. Together, these proteins might confer salt tolerance in Pokkali by maintaining a low cytosolic Na+ level and a correct ratio of cytosolic Na+/K+.

Keywords: Cytosolic Na+/K+ homeostasis; FRET efficiency; NaCl stress; OsHKT1; OsHKT2; OsVHA; vacuole volumes.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Exp BotHome page
S. W. Ardie, L. Xie, R. Takahashi, S. Liu, and T. Takano
Cloning of a high-affinity K+ transporter gene PutHKT2;1 from Puccinellia tenuiflora and its functional comparison with OsHKT2;1 from rice in yeast and Arabidopsis
J. Exp. Bot., August 1, 2009; 60(12): 3491 - 3502.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Jabnoune, S. Espeout, D. Mieulet, C. Fizames, J.-L. Verdeil, G. Conejero, A. Rodriguez-Navarro, H. Sentenac, E. Guiderdoni, C. Abdelly, et al.
Diversity in Expression Patterns and Functional Properties in the Rice HKT Transporter Family
Plant Physiology, August 1, 2009; 150(4): 1955 - 1971.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
H. J. Kronzucker, M. W. Szczerba, L. M. Schulze, and D. T. Britto
Non-reciprocal interactions between K+ and Na+ ions in barley (Hordeum vulgare L.)
J. Exp. Bot., July 1, 2008; 59(10): 2793 - 2801.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. Huang, W. Spielmeyer, E. S. Lagudah, and R. Munns
Comparative mapping of HKT genes in wheat, barley, and rice, key determinants of Na+ transport, and salt tolerance
J. Exp. Bot., March 5, 2008; (2008) ern033v1.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
R. Takahashi, S. Liu, and T. Takano
Cloning and functional comparison of a high-affinity K+ transporter gene PhaHKT1 of salt-tolerant and salt-sensitive reed plants
J. Exp. Bot., December 1, 2007; 58(15-16): 4387 - 4395.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.