Skip Navigation



JXB Advance Access published online on November 22, 2006
Journal of Experimental Botany, doi:10.1093/jxb/erl218
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrowOA All Versions of this Article:
58/3/473    most recent
erl218v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Qin, Y.-M.
Right arrow Articles by Zhu, Y.-X.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Qin, Y.-M.
Right arrow Articles by Zhu, Y.-X.
Agricola
Right arrow Articles by Qin, Y.-M.
Right arrow Articles by Zhu, Y.-X.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2006 The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Received June 19, 2006
Revised September 19, 2006
Accepted September 28, 2006

RESEARCH PAPER

Genetic and biochemical studies in yeast reveal that the cotton fibre-specific GhCER6 gene functions in fatty acid elongation

Yong-Mei Qin 1 *, François M. Pujol 2, Chun-Yang Hu 1, Jian-Xun Feng 1, Alexander J. Kastaniotis 2, J. Kalervo Hiltunen 2, and Yu-Xian Zhu 1

1 National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, Beijing 100871, China; Department of Biochemistry and Molecular Biology, College of Life Sciences, Peking University, Beijing 100871, China
2 Biocenter Oulu and Department of Biochemistry, University of Oulu, PO Box 3000, FIN-90014, Finland

* To whom correspondence should be addressed.
Yong-Mei Qin, E-mail: qinym{at}pku.edu.cn


  Abstract

3-Ketoacyl-CoA synthase catalyses the initial condensation reaction during fatty acid elongation using malonyl-CoA and long-chain acyl-CoA as substrates. Previously, it was reported that several genes encoding putative cotton 3-ketoacyl-CoA synthases were significantly up-regulated during early cotton fibre development. In this study, GhCER6 cDNA that contains an open reading frame of 1479 bp, encoding a protein of 492 amino acid residues homologous to the Arabidopsis condensing enzyme CER6, was isolated and cloned. In situ hybridization results demonstrated that GhCER6 mRNA was detected only in the elongating wild-type cotton fibre cells. When GhCER6 was transformed to the Saccharomyces cerevisiae elo3 deletion mutation strain that was deficient in the production of 26-carbon fatty acids and displayed a very slow-growth phenotype, the mutant cells were found to divide similarly compared with those of the wild-type cells. Further, heterologous expression of GhCER6 restored the viability of the S. cerevisiae haploid elo2 and elo3 double-deletion strain. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis showed that GhCER6 was enzymatically active since the yeast elo2 and elo3 double-deletion mutant expressing the cotton gene produced very-long-chain fatty acids that are essential for cell growth. The results suggest that GhCER6 encodes a functional 3-ketoacyl-CoA synthase.

Keywords: Fatty acid elongation; Gossypium hirsutum; 3-ketoacyl-CoA synthase; MALDI-TOF-MS.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Exp BotHome page
X.-R. Li, L. Wang, and Y.-L. Ruan
Developmental and molecular physiological evidence for the role of phosphoenolpyruvate carboxylase in rapid cotton fibre elongation
J. Exp. Bot., October 8, 2009; (2009) erp299v1.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
W.-Q. Song, Y.-M. Qin, M. Saito, T. Shirai, F. M. Pujol, A. J. Kastaniotis, J. K. Hiltunen, and Y.-X. Zhu
Characterization of two cotton cDNAs encoding trans-2-enoyl-CoA reductase reveals a putative novel NADPH-binding motif
J. Exp. Bot., April 1, 2009; 60(6): 1839 - 1848.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
O. Serra, M. Soler, C. Hohn, R. Franke, L. Schreiber, S. Prat, M. Molinas, and M. Figueras
Silencing of StKCS6 in potato periderm leads to reduced chain lengths of suberin and wax compounds and increased peridermal transpiration
J. Exp. Bot., February 1, 2009; 60(2): 697 - 707.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
X.-C. He, Y.-M. Qin, Y. Xu, C.-Y. Hu, and Y.-X. Zhu
Molecular cloning, expression profiling, and yeast complementation of 19 {beta}-tubulin cDNAs from developing cotton ovules
J. Exp. Bot., July 1, 2008; 59(10): 2687 - 2695.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Mintz-Oron, T. Mandel, I. Rogachev, L. Feldberg, O. Lotan, M. Yativ, Z. Wang, R. Jetter, I. Venger, A. Adato, et al.
Gene Expression and Metabolism in Tomato Fruit Surface Tissues
Plant Physiology, June 1, 2008; 147(2): 823 - 851.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
J. J. Lee, A. W. Woodward, and Z. J. Chen
Gene Expression Changes and Early Events in Cotton Fibre Development
Ann. Bot., December 1, 2007; 100(7): 1391 - 1401.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
B. Luo, X.-Y. Xue, W.-L. Hu, L.-J. Wang, and X.-Y. Chen
An ABC Transporter Gene of Arabidopsis thaliana, AtWBC11, is Involved in Cuticle Development and Prevention of Organ Fusion
Plant Cell Physiol., December 1, 2007; 48(12): 1790 - 1802.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
Y.-M. Qin, C.-Y. Hu, Y. Pang, A. J. Kastaniotis, J. K. Hiltunen, and Y.-X. Zhu
Saturated Very-Long-Chain Fatty Acids Promote Cotton Fiber and Arabidopsis Cell Elongation by Activating Ethylene Biosynthesis
PLANT CELL, November 1, 2007; 19(11): 3692 - 3704.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.