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JXB Advance Access published online on March 5, 2007
Journal of Experimental Botany, doi:10.1093/jxb/erm014
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© The Author [2007]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

RESEARCH PAPER

OsMTN encodes a 5'-methylthioadenosine nucleosidase that is up-regulated during submergence-induced ethylene synthesis in rice (Oryza sativa L.)

Guillaume Rzewuski1, Kenneth A. Cornell2, Lee Rooney2, Katharina Bürstenbinder1, Markus Wirtz3, Rüdiger Hell3 and Margret Sauter1,*

1Botanisches Institut, Universität Kiel, Am Botanischen Garten 1–9, D-24118 Kiel, Germany
2Department of Chemistry, Boise State University, 1910 University Drive, Boise, ID 83725-1520, USA
3Heidelberg Institute of Plant Sciences (HIP), University of Heidelberg, Im Neuenheimer Feld 360, D-69120 Heidelberg, Germany

* To whom correspondence should be addressed. E-mail: msauter{at}bot.uni-kiel.de

Methylthioadenosine (MTA) is released as a by-product of S-adenosylmethionine (AdoMet)-dependent reactions central to ethylene, polyamine, or phytosiderophore biosynthesis. MTA is hydrolysed by methylthioadenosine nucleosidase (MTN; EC 3.2.2.16 [EC] ) into adenine and methylthioribose which is processed through the methionine (Met) cycle to produce a new molecule of AdoMet. In deepwater rice, submergence enhances ethylene biosynthesis, and ethylene in turn influences the methionine cycle through positive feedback regulation of the acireductone dioxygenase gene OsARD1. In rice, MTN is encoded by a single gene designated OsMTN. Recombinant OsMTN enzyme had a KM for MTA of 2.1 mM and accepted a wide array of 5' substitutions of the substrate. OsMTN also metabolized S-adenosylhomocysteine (AdoHcy) with 15.9% the rate of MTA. OsMTN transcripts and OsMTN-specific activity increased slowly and in parallel upon submergence, indicating that regulation occurred mainly at the transcriptional level. Neither ethylene, MTA, nor Met regulated OsMTN expression. Analysis of steady-state metabolite levels showed that MTN activity was sufficiently high to prevent Met and AdoMet depletion during long-term ethylene biosynthesis.

Key words: Methylthioadenosine nucleosidase, sulphur metabolism

Received 25 October 2006; Revised 11 January 2007 Accepted 17 January 2007


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