Skip Navigation



JXB Advance Access published online on April 2, 2007
Journal of Experimental Botany, doi:10.1093/jxb/erm030
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
58/7/1753    most recent
erm030v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Barthod, S.
Right arrow Articles by Epron, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Barthod, S.
Right arrow Articles by Epron, D.
Agricola
Right arrow Articles by Barthod, S.
Right arrow Articles by Epron, D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author [2007]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

RESEARCH PAPER

Can dual chlorophyll fluorescence excitation be used to assess the variation in the content of UV-absorbing phenolic compounds in leaves of temperate tree species along a light gradient?

Sandrine Barthod1, Zoran Cerovic2 and Daniel Epron3,*

1Université de Franche-Comté, Laboratoire de Biologie Environnementale, Pôle de Montbéliard, 4 place Tharradin, BP 71427, F-25 211 Montbéliard Cedex, France
2Equipe de Biospectroscopie Végétale, Département d' Écophysiologie Végétale, Laboratoire d'Ecologie Systémique et Evolution, Université de Paris Sud, Bâtiment 362, F-91405 Orsay Cedex, France
3Nancy University, Université Henri Poincaré, UMR 1137 INRA–UHP Ecologie et Ecophysiologie Forestières, BP 239, F-54506 Vandoeuvre Cedex, France

* To whom correspondence should be addressed. E-mail: daniel.epron{at}scbiol.uhp-nancy.fr

The present study assesses light-induced variations in phenolic compounds in leaves of saplings of two co-occurring temperate species (Acer platanoides L., and Fraxinus excelsior L.) along a light gradient using a new non-invasive optical method (Dualex). The Dualex-derived UV absorbance of leaf epidermis (the sum of the adaxial and abaxial faces, AUV) increased significantly with increasing light in both species. AUV values were correlated with absorbance of the leaf extract at 305 nm and 375 nm (A305 and A375) in both species with similar slopes for both species. However, a large difference in intercept was observed between the two species when A305 was regressed against AUV. Similarly, AUV values were well correlated with the amount of phenolics in the leaf extracts assessed by the Folin–Ciocalteu method, but slopes were significantly different for the two species. Thus, the UV-A epidermal transmittance, despite being a reliable indicator of the UV-screening capacity of the leaf epidermis, cannot be used for any quantitative estimate of UV-B screening capacity or of energetic requirement for leaf construction without a species-specific calibration.

Key words: Acer platanoides L., epidermis, fluorescence, Fraxinus excelsior L., phenolic compounds, UV absorbance, shade

Received 22 September 2006; Revised 20 January 2007 Accepted 30 January 2007


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Exp BotHome page
M. N. Merzlyak, T. B. Melo, and K. R. Naqvi
Effect of anthocyanins, carotenoids, and flavonols on chlorophyll fluorescence excitation spectra in apple fruit: signature analysis, assessment, modelling, and relevance to photoprotection
J. Exp. Bot., February 5, 2008; (2008) erm316v1.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.