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JXB Advance Access published online on June 11, 2007
Journal of Experimental Botany, doi:10.1093/jxb/erm123
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© 2007 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

RESEARCH PAPER

Isolation and comparative analysis of the wheat TaPT2 promoter: identification in silico of new putative regulatory motifs conserved between monocots and dicots

A Tittarelli1 *, L Milla2 *, F Vargas1,2, A Morales1, C Neupert2, LA Meisel1, H Salvo-G3, E Peñaloza3, G Muñoz3, LJ Corcuera4 and H Silva1,{dagger}

1Millennium Nucleus in Plant Cell Biology and Center of Plant Biotechnology, Andres Bello University, Av. República 217, 837-0146, Santiago, Chile
2Laboratorio de Genética Molecular Vegetal, Facultad de Ciencias, Universidad de Chile, Las Palmeras 3425, Ñuñoa, Santiago, Chile
3Instituto de Investigaciones Agropecuarias, Unidad de Biotecnología, INIA-Carillanca, Casilla 58-D, Temuco, Chile
4Departamento de Botánica, Universidad de Concepción, Casilla 160-C, Concepción, Chile

{dagger} To whom correspondence should be addressed. E-mail: hsilva{at}unab.cl

Phosphorus deficiency is one of the major nutrient stresses affecting plant growth. Plants respond to phosphate (Pi) deficiency through multiple strategies, including the synthesis of high-affinity Pi transporters. In this study, the expression pattern of one putative wheat high-affinity phosphate transporter, TaPT2, was examined in roots and leaves under Pi-deficient conditions. TaPT2 transcript levels increased in roots of Pi-starved plants. A 579 bp fragment of the TaPT2 promoter is sufficient to drive the expression of the GUS reporter gene specifically in roots of Pi-deprived wheat. This TaPT2 promoter fragment was also able to drive expression of the GUS reporter gene in transgenic Arabidopsis thaliana, under similar growth conditions. Conserved regions and candidate regulatory motifs were detected by comparing this promoter with Pi transporter promoters from barley, rice, and Arabidopsis. Altogether, these results indicate that there are conserved cis-acting elements and trans-acting factors that enable the TaPT2 promoter to be regulated in a tissue-specific and Pi-dependent fashion in both monocots and dicots.

Key words: Arabidopsis thaliana, high affinity phosphate transporter, promoter, Triticum aestivum

* These authors contributed equally to this work.

Received 5 February 2007; Revised 30 April 2007 Accepted 1 May 2007


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