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JXB Advance Access published online on July 3, 2007
Journal of Experimental Botany, doi:10.1093/jxb/erm140
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© 2007 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

RESEARCH PAPER

Targeted transcript mapping for agronomic traits in potato

Asun Fernández-del-Carmen* {dagger}, Carolina Celis-Gamboa, Richard G. F. Visser and Christian W. B. Bachem

Wageningen University and Research Centrum, Department of Plant Sciences, Laboratory of Plant Breeding, PO Box 386, 6700 AJ Wageningen, The Netherlands

* To whom correspondence should be addressed. E-mail: maferde3{at}ibmcp.upv.es

A combination of cDNA-amplified fragment length polymorphism (AFLP) and bulked segregant analysis (BSA) was used to identify genes co-segregating with earliness of tuberization in a diploid potato population. This approach identified 37 transcript-derived fragments with a polymorphic segregation pattern between early and late tuberizing bulks. Most of the identified transcripts mapped to chromosomes 5 (19 markers) and 12 (eight markers) of the paternal map. Quantitative trait locus (QTL) mapping of tuberization time also identified earliness QTLs on these two chromosomes. A potato bacterial artificial chromosome (BAC) library was screened with four of the markers linked to the main QTL. BAC contigs containing the markers showing the highest association with the trait have been identified. One of these contigs has been anchored to chromosome 5 on an ultradense genetic map of potato, which could be used as a starting point for map-based cloning of genes associated with earliness.

Key words: Bulked segregant analysis, cDNA-AFLP, potato tuberization, QTL, transcript mapping

{dagger} Present address: Instituto de Biología Molecular y Celular de Plantas, Avenida Los Naranjos s/n, 46022 Valencia, Spain.

Received 5 January 2007; Revised 21 April 2007 Accepted 29 May 2007


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