Skip Navigation



JXB Advance Access published online on August 13, 2008
Journal of Experimental Botany, doi:10.1093/jxb/ern202
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrowOA All Versions of this Article:
59/13/3523    most recent
ern202v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Hashimoto, K.
Right arrow Articles by Yokota, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hashimoto, K.
Right arrow Articles by Yokota, E.
Agricola
Right arrow Articles by Hashimoto, K.
Right arrow Articles by Yokota, E.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2008 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

RESEARCH PAPER

An isoform of Arabidopsis myosin XI interacts with small GTPases in its C-terminal tail region

Kohsuke Hashimoto1, Hisako Igarashi2, Shoji Mano3, Chikako Takenaka4, Takashi Shiina4, Masatoshi Yamaguchi2, Taku Demura2, Mikio Nishimura3, Teruo Shimmen1 and Etsuo Yokota1,*

1Department of Life Science, Graduate School of Life Science, University of Hyogo, Harima Science Park City, Hyogo 678-1279, Japan
2RIKEN Plant Science Center, 1-7-22 Suehiro-cho Turumi-ku, Yokohama, Kanagawa 230-0045, Japan
3Department of Cell Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan
4Graduate School of Human Environmental Sciences, Kyoto Prefectural University, Nakaragi-cho, Shimogamo, Sakyo-ku, Kyoto 606-8522, Japan

* To whom correspondence should be addressed: E-mail: yokota{at}sci.u-hyogo.ac.jp

Myosin XI, a class of myosins expressed in plants is believed to be responsible for cytoplasmic streaming and the translocation of organelles and vesicles. To gain further insight into the translocation of organelles and vesicles by myosin XI, an isoform of Arabidopsis myosin XI, MYA2, was chosen and its role in peroxisome targeting was examined. Using the yeast two-hybrid screening method, two small GTPases, AtRabD1 and AtRabC2a, were identified as factors that interact with the C-terminal tail region of MYA2. Both recombinant AtRabs tagged with His bound to the recombinant C-terminal tail region of MYA2 tagged with GST in a GTP-dependent manner. Furthermore, AtRabC2a was localized on peroxisomes, when its CFP-tagged form was expressed transiently in protoplasts prepared from Arabidopsis leaf tissue. It is suggested that MYA2 targets the peroxisome through an interaction with AtRabC2a.

Key words: AtRabC2a, AtRabD1, myosin XI, MYA2, peroxisome

Received 6 March 2008; Revised 7 July 2008 Accepted 8 July 2008


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
D. Avisar, M. Abu-Abied, E. Belausov, E. Sadot, C. Hawes, and I. A. Sparkes
A Comparative Study of the Involvement of 17 Arabidopsis Myosin Family Members on the Motility of Golgi and Other Organelles
Plant Physiology, June 1, 2009; 150(2): 700 - 709.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
G. Cai and M. Cresti
Organelle motility in the pollen tube: a tale of 20 years
J. Exp. Bot., February 1, 2009; 60(2): 495 - 508.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.