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JXB Advance Access published online on June 24, 2009
Journal of Experimental Botany, doi:10.1093/jxb/erp172
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© 2009 The Author(s).
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

RESEARCH PAPER

Remobilization of leaf S compounds and senescence in response to restricted sulphate supply during the vegetative stage of oilseed rape are affected by mineral N availability

L. Dubousset1, M. Abdallah1, A. S. Desfeux1, P. Etienne1, F. Meuriot1, M. J. Hawkesford2, J. Gombert1, R. Ségura1, M-P. Bataillé1, S. Rezé1, J. Bonnefoy1, A. F. Ameline1, A. Ourry1, F. Le Dily1 and J. C. Avice1,*

1INRA, UMR INRA-UCBN 950 Ecophysiologie Végétale, Agronomie et Nutritions NCS, Esplanade de la Paix, F-14032, Caen, France
2Plant Science Department, Rothamsted Research, Harpenden, Herts AL5 2JQ, UK

* To whom correspondence should be addressed. E-mail: jean-christophe.avice{at}unicaen.fr

The impact of sulphur limitation on the remobilization of endogenous S compounds during the rosette stage of oilseed rape, and the interactions with N availability on these processes, were examined using a long-term 34SOFormula labelling method combined with a study of leaf senescence progression (using SAG12/Cab as a molecular indicator) and gene expression of the transporters, BnSultr4;1 and BnSultr4;2, involved in vacuolar sulphate efflux. After 51 d on hydroponic culture at 0.3 mM 34SOFormula (1 atom% excess), the labelling was stopped and plants were subject for 28 d to High S-High N (HS-HN, control), Low S-High N (LS-HN) or Low S-Low N (LS-LN) conditions. Compared with the control, LS-HN plants showed delayed leaf senescence and, whilst the shoot growth and the foliar soluble protein amounts were not affected, S, 34S, and SOFormula amounts in the old leaves declined rapidly and were associated with the up-regulation of BnSultr4;1. In LS-LN plants, shoot growth was reduced, leaf senescence was accelerated, and the rapid S mobilization in old leaves was accompanied by decreased 34S and SOFormula, higher protein mobilization, and up-regulation of BnSultr4;2, but without any change of expression of BnSultr4;1. The data suggest that to sustain the S demand for growth under S restriction (i) vacuolar SOFormula is specifically remobilized in LS-HN conditions without any acceleration of leaf senescence, (ii) SOFormula mobilization is related to an up-regulation of BnSultr4;1 and/or BnSultr4;2 expression, and (iii) the relationship between sulphate mobilization and up-regulation of expression of BnSultr4 genes is specifically dependent on the N availability.

Key words: Brassica napus, Group 4 sulphate transporters, leaf senescence, S compounds recycling, 34SOFormula labelling, sulphur limitation

Received 3 February 2009; Revised 21 April 2009 Accepted 27 April 2009


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