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JXB Advance Access published online on June 24, 2009
Journal of Experimental Botany, doi:10.1093/jxb/erp205
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© The Author [2009]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

RESEARCH PAPER

Identification of leaf proteins differentially accumulated during cold acclimation between Festuca pratensis plants with distinct levels of frost tolerance

Arkadiusz Kosmala1,*, Aleksandra Bocian1, Marcin Rapacz2, Barbara Jurczyk2 and Zbigniew Zwierzykowski1

1Institute of Plant Genetics, Polish Academy of Sciences, Strzeszynska 34, 60-479 Poznan, Poland
2University of Agriculture in Krakow, Faculty of Agriculture and Economics, Department of Plant Physiology, Podluzna 3, 30-239 Cracow, Poland

* To whom correspondence should be addressed: E-mail: akos{at}igr.poznan.pl

Festuca pratensis (meadow fescue) as the most frost-tolerant species within the Lolium–Festuca complex was used as a model for research aimed at identifying the cellular components involved in the cold acclimation (CA) of forage grasses. The work presented here also comprises the first comprehensive proteomic research on CA in a group of monocotyledonous species which are able to withstand winter conditions. Individual F. pratensis plants with contrasting levels of frost tolerance, high frost tolerant (HFT) and low frost tolerant (LFT) plants, were selected for comparative proteomic research. The work focused on the analysis of leaf protein accumulation before and after 2, 8, and 26 h, and 3, 5, 7, 14, and 21 d of CA, using high-throughput two-dimensional electrophoresis, and on the identification of proteins which were accumulated differentially between the selected plants by the application of mass spectrometry. The analyses of approximately 800 protein profiles revealed a total of 41 (5.1%) proteins that showed a minimum of a 1.5-fold difference in abundance, at a minimum of one time point of CA for HFT and LFT genotypes. It was shown that significant differences in profiles of protein accumulation between the analysed plants appeared relatively early during cold acclimation, most often after 26 h (on the 2nd day) of CA and one-half of the differentially accumulated proteins were all parts of the photosynthetic apparatus. Several proteins identified here have been reported to be differentially accumulated during cold conditions for the first time in this paper. The functions of the selected proteins in plant cells and their probable influence on the level of frost tolerance in F. pratensis, are discussed.

Key words: Cold acclimation, 2-D electrophoresis, expression profiles, Festuca pratensis, frost tolerance, grasses, low temperature stress, mass spectrometry, protein abundance, proteome

Received 16 April 2009; Revised 21 May 2009 Accepted 31 May 2009


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